Abstract
Objectives Long non-coding RNAs (lncRNAs) have been identified as key regulators in the development of atherosclerosis, which is a major cause of ischemic stroke. However, to date, there are no reports on the association between lncRNA gene variation and the risk of ischemic stroke. Therefore, we assessed the association between H19 and MALAT1 gene polymorphisms and susceptibility to ischemic stroke in a northern Chinese Han population. Methods In our study, we genotyped four genetic variations in lncRNA-H19 and -MALAT1 (rs217727, rs2251375, rs619586, and rs3200401) in a case–control study of 567 ischemic stroke patients and 552 control subjects. Results We found that the TT genotype of the rs217727 polymorphism within H19 was significantly associated with increased risk of ischemic stroke in our northern Chinese Han population (odds ration (OR) = 1.519, 95% confidence interval (CI) = 1.072–2.152, p = 0.018). Stratified analysis based on stroke subtype revealed that the increased risk was more evident in small vessel ischemic stroke (OR = 1.941, 95% CI = 1.260–2.992, p = 0.02). Individuals with the TT genotype had a 1.941 times higher risk of small vessel ischemic stroke when compared with the subjects of CC + CT. These correlations remained after adjusting for confounding risk factors of stroke (OR = 1.913, 95% CI = 1.221–2.998, p = 0.005). However, there was no significant association between H19 rs2251375 or MALAT1 rs3200401 and ischemic stroke in either total population analysis or subgroup analysis. Conclusion In conclusion, our findings suggest that the H19 rs217727 gene polymorphism contributes to small vessel ischemic stroke susceptibility in the Chinese Han population and may serve as a potential indicator for ischemic stroke susceptibility.
Highlights
Ischemic stroke (IS) is a major threat to health and quality of life in modern society [1]
In recent years, increasing evidence suggests that new candidate markers long non-coding RNAs, which are functional RNA molecules that are not translated into proteins, contribute to atherosclerotic-related disease [3]
According to TOAST typing, patients were classified into two subtypes: large-artery atherosclerosis (LAA) and small-vessel occlusive (SVO) stroke, while other subtypes were excluded from this study [22]
Summary
Ischemic stroke (IS) is a major threat to health and quality of life in modern society [1]. Studies have identified a series of new candidate gene loci that may contribute to the pathogenesis of IS [2]. In recent years, increasing evidence suggests that new candidate markers long non-coding RNAs (lncRNAs), which are functional RNA molecules that are not translated into proteins, contribute to atherosclerotic-related disease [3]. Research into lncRNAs in IS is just beginning. Non-coding RNA (ncRNA) can be classified into short and long ncRNAs. LncRNAs are a subclass of non-coding RNAs with lengths of more than 200 nucleotides, and lack protein-encoding capacity. LncRNAs are a subclass of non-coding RNAs with lengths of more than 200 nucleotides, and lack protein-encoding capacity They are thought to function through a variety of mechanisms, such as in transcription, translation, genome rearrangement, and chromatin modification [4].
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