Abstract
Renal cell carcinoma (RCC) is a malignancy with a poor prognosis. We aimed to explore whether the expression of Long Non-Coding RNA (LncRNA) growth arrest-specific transcript 5 (GAS5) is associated with RCC genesis. We selected twelve clinical samples diagnosed for renal clear cell carcinoma and found that the LncRNA GAS5 transcript levels were significantly reduced relative to those in adjacent unaffected normal renal tissues. In addition, expression of GAS5 was lower in the RCC cell line A498 than that in normal renal cell line HK-2. Furthermore, using functional expression cloning, we found that overexpression of GAS5 in A498 cells inhibited cell proliferation, induced cell apoptosis and arrested cell cycling. At the same time, the migration and invasion potential of A498 cells were inhibited compared to control groups. Our study provided the first evidence that a decrease in GAS5 expression is associated with RCC genesis and progression and overexpression of GAS5 can act as a tumor suppressor for RCC, providing a potential attractive therapeutic approach for this malignancy.
Highlights
Renal cell carcinoma (RCC) accounts for approximately 3% of adult malignancies and more than 90% of renal cancers (Chow et al, 1999)
We aimed to explore whether the expression of Long Non-Coding RNA (LncRNA) growth arrest-specific transcript 5 (GAS5) is associated with RCC genesis
Using functional expression cloning, we found that overexpression of GAS5 in A498 cells inhibited cell proliferation, induced cell apoptosis and arrested cell cycling
Summary
Renal cell carcinoma (RCC) accounts for approximately 3% of adult malignancies and more than 90% of renal cancers (Chow et al, 1999) It is the third most common urological cancer after prostate and bladder cancer, but it has the highest mortality rate at over 40% (van Spronsen et al, 2005). Accumulating data show that many identified LncRNAs are crucial players in a variety of tissue carcinogenesis, invasion, and metastasis (Huarte and Rinn, 2010; Tsai et al, 2011). According to their functions, LncRNAs can be roughly divided into oncogenic and tumor-suppressor groups.
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