Long non‐coding RNA changed in expression in tumors growing on EP2 knockout mice (711.4)

Abstract

Cyclooxygenase‐2 and prostaglandin E2 (PGE2) displayed increased expression in tumor tissue and promotes tumor progression. PGE2 exerts its action via four membrane receptors, EP1‐4, and patients with high EP2 receptor expression have worse prognosis. Accordingly, tumors growing on EP2 knockout mice display reduced tumor weight compared to wild type mice. Therefore, our aim was to evaluate the effect of EP2 on tumor growth by analyses of differences in RNA expression between tumors in EP2 knockout and wild type mice with gene expression arrays (Sureprint, Agilent). Results displayed that several long non‐coding RNA (lncRNA) had a more than 5‐fold decreased expression in tumor tissue at host EP2 knockout and more than 100 had a 2‐fold change. Many lncRNAs are not characterized and their function is unknown. However, lncRNAs are known to regulate transcription and expression of genes. Therefore, our observations on lncRNA expression may be involved in tumor growth regulation.Grant Funding Source: Supported by the Swedish Cancer Society (CAN 2010/255) and the Assar Gabrielsson foundation