Long noncoding RNA CASC9.5 promotes the proliferation and metastasis of lung adenocarcinoma

Jianlong Zhou,Weimin Zhang,Guibin Qiao,Yu Zhong,Haiping Xiao,Hao Tian,Zhiyong Xu,Limin Ma,Jing Liang,Xinglong Yang

doi:10.1038/s41598-017-18280-3

Abstract

Long non-coding RNAs (lncRNAs) are important regulatory factors in tumor development and progression. The lncRNA CASC9.5 is located on chromosome 8 and has a total length of 1316 bp. CASC9.5 plays a tumor-promoting role in the development and progression of brain tumor and colon cancer; however, limited research has been conducted on the role of this lncRNA in lung adenocarcinoma. The present study analyzed 44 lung adenocarcinoma specimens and 2 lung cancer cell lines. It was found that CASC9.5 expression levels were significantly higher in lung cancer tissues and cells compared with normal lung tissues. In addition, the expression level of CASC9.5 was closely related to the TNM (tumor, node and metastasis) stage of lung adenocarcinomas, tumor size, tumor metastasis and tumor metabolism. Moreover, results of the in vivo and in vitro experiments all demonstrated that CASC9.5 promoted lung adenocarcinoma cell proliferation and metabolism by regulating the expression levels of cyclin D1, E-cadherin, N-cadherin and β-catenin. In summary, the present study demonstrated that high levels of CASC9.5 expression promote the proliferation, metastasis and metabolism of lung adenocarcinoma cells and might serve as a prognostic indicator. The present study provides novel findings regarding the diagnosis and treatment of lung adenocarcinoma.

Highlights

At present, lung cancer is the most common primary malignancy of the lung
The long non-coding RNAs (lncRNAs) CASC9.5 is located on chromosome 8 and has a length of 1316 bp
Our results suggested that high levels of CASC9.5 expression promote the proliferation, metastasis and metabolism of lung adenocarcinoma cells, CASC9.5 would serve as a prognostic indicator due to its important role in lung adenocarcinoma

Summary

Introduction

Lung cancer is the most common primary malignancy of the lung. The vast majority of lung cancers originate from the epithelium of the bronchial mucosa. Recent studies indicate that long non-coding RNAs (lncRNAs) may be related to the pathogenesis of non-small cell lung cancer (NSCLC)[5,6] These studies have provided a new idea for the investigation of the molecular mechanisms underlying the metastasis and metabolism of lung adenocarcinoma. Studies have shown that abnormal regulation of lncRNAs is related to the development and metabolism of a variety of cancers, including lung cancer[11,12,13] Such studies provide a comprehensive understanding of the important biological role of the EMT in the development and metabolism of lung adenocarcinoma. Our results indicate that increased expression of CASC9.5 in lung adenocarcinoma may play an important role in the growth and metastasis of cancer cells. Our results suggested that high levels of CASC9.5 expression promote the proliferation, metastasis and metabolism of lung adenocarcinoma cells, CASC9.5 would serve as a prognostic indicator due to its important role in lung adenocarcinoma

Methods

Results

Conclusion