Abstract
Emerging evidence has classified the aberrant expression of long non‐coding RNAs (lncRNAs) as a basic signature of various malignancies including gastric cancer (GC). LINC01225 has been shown to act as a hepatocellular carcinoma‐related gene, with its expression pattern and biological function not clarified in GC. Here, we verified that LINC01225 was up‐regulated in tumour tissues and plasma of GC. Analysis with clinicopathological information suggested that up‐regulation of LINC01225 was associated with advanced disease and poorer overall survival. Receiver operating characteristic (ROC) analysis showed that plasma LINC01225 had a moderate accuracy for diagnosis of GC. In addition, knockdown of LINC01225 led to retardation of cell proliferation, invasion and migration, and overexpression of LINC01225 showed the opposite effects. Mechanistic investigations showed that LINC01225 silencing inhibited epithelial‐mesenchymal transition (EMT) process and attenuated Wnt/β‐catenin signalling of GC. Furthermore, ectopic expression of Wnt1 or suppression of GSK‐3β abolished the si‐LINC01225‐mediated suppression against EMT, thereby promoting cell proliferation, invasion and migration of GC. In conclusion, LINC01225 promotes the progression of GC through Wnt/β‐catenin signalling pathway, and it may serve as a potential target or strategy for diagnosis or treatment of GC.
Highlights
Gastric cancer (GC) is the fifth most frequently diagnosed cancer and the third leading cause of cancer death worldwide.[1]
Wang et al first reported that LINC01225 acted as a hepatocellular carcinoma (HCC)‐related ln‐ cRNA and that its high expression was significantly associated with advanced TNM stage, larger tumour size and positive lymph node metastasis.11Specifically, they found that LINC01225 promoted HCC growth and invasion via epidermal growth factor receptor/mi‐ togen‐activated protein kinase (EGFR/MAPK) pathway.[11]
LncRNAs have emerged as critical players in tumorigenesis and can‐ cer progression; the potential roles and mechanistic details for most Long non‐coding RNAs (lncRNAs) still remain unclear in gastric cancer (GC)
Summary
Gastric cancer (GC) is the fifth most frequently diagnosed cancer and the third leading cause of cancer death worldwide.[1]. | 7582 non‐coding RNAs (ncRNAs).[3] Long non‐coding RNAs (lncRNAs) are a class of ncRNAs that transcribe longer than 200 nucleotides, with limited or no protein‐coding capacity.[4] Once considered as tran‐ scriptional noise, lncRNAs have recently been implicated in a wide range of biological processes including normal tissue development, regulation of cellular pluripotency, modulation of cell proliferation and apoptosis. Located at chromosome 1p35.2, LINC01225 is a lincRNA with a length of 2113 base pairs (bps).[11] Wang et al first reported that LINC01225 acted as a hepatocellular carcinoma (HCC)‐related ln‐ cRNA and that its high expression was significantly associated with advanced TNM stage, larger tumour size and positive lymph node metastasis.11Specifically, they found that LINC01225 promoted HCC growth and invasion via epidermal growth factor receptor/mi‐ togen‐activated protein kinase (EGFR/MAPK) pathway.[11] the role of LINC01225 in other cancers including GC is unknown up to now and remains to be elucidated. Our findings support an oncogenic role of LINC01225 in GC progres‐ sion and suggest that LINC01225 may be served as a candidate bio‐ marker for diagnosis, staging and outcome prediction of GC
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