Abstract
BackgroundLong intergenic non-protein coding RNA 520 (LINC00520), a novel identified lncRNA, has been shown to modulate the malignant phenotype of tumor cells in some malignant tumors. However, the exact role and molecular mechanism of LINC00520 in malignant melanoma has not been studied.MethodsThe expression of LINC00520 in melanoma tissues were detected by using RNA-seq analysis and qRT-PCR. Melanoma cases from the public databases (The Cancer Genome Atlas (TCGA), GEO#GSE15605, GEO#GSE34460 and GEO#GSE24996) were included in this study. CCK-8 assay, EdU assay, transwell and scratch wound assay were used to explore the role of LINC00520 in melanoma cells. Luciferase reporter assays, MS2-RIP, RNA pull-down and RNA-ChIP assay were used to demonstrate the molecular biological mechanism of LINC00520 in melanoma.ResultsWe found that LICN00520 was found to be overexpressed in melanoma tissue. High expression of LICN00520 is a risk factor for the prognosis of melanoma patients. LINC00520 promotes the proliferation, invasion and migration of melanoma cells. LICN00520 exerted its oncogenic role by competitive binding miR-125b-5p to promote Eukaryotic initiation factor 5A2 (EIF5A2) expression. We also showed that LICN00520 promotes the growth and metastasis of melanoma in vivo through regulating miR-125b-5p/EIF5A2 axis.ConclusionsAll results elucidated the role and molecular mechanism of LINC00520 in the malignant development of melanoma. LINC00520, a new oncogene in melanoma, maybe serve as a survival biomarkers or therapeutic target for melanoma patients.
Highlights
Malignant melanoma is the most dangerous skin tumor, which is the primary cause of death of skin cancer [1,2,3]
We proved that miR-125b-5p exerts anti-cancer effects in melanoma by targeting Eukaryotic initiation factor 5A2 (EIF5A2)
LINC00520 was significantly up-regulated in melanoma We first analysed the Long non-coding RNAs (lncRNAs) expression profiling in three malignant melanoma tissues and three adjacent normal tissues (ANT) by using RNA-seq
Summary
Malignant melanoma is the most dangerous skin tumor, which is the primary cause of death of skin cancer [1,2,3]. Long non-coding RNAs (lncRNAs), a kind of non-coding RNA over 200 nucleotides in length, play pivotal roles in various human tumors by modulating the malignant phenotype of tumor cells [7,8,9]. Long intergenic non-protein coding RNA 520 (LINC00520), located on chromosome 14, has been reported to overexpress and function as a oncogene in breast cancer, nasopharyngeal carcinoma and laryngeal squamous cell carcinoma [12,13,14]. Long intergenic non-protein coding RNA 520 (LINC00520), a novel identified lncRNA, has been shown to modulate the malignant phenotype of tumor cells in some malignant tumors. The exact role and molecular mechanism of LINC00520 in malignant melanoma has not been studied
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
