Abstract
Background and aimThe biological functions of non-coding RNAs (ncRNAs) have been widely identified in many human diseases. In the present study, the relationship between long non-coding RNA HOTAIR and microRNA-17-5p (miR-17-5p) and their roles in osteogenic differentiation and proliferation in non-traumatic osteonecrosis of femoral head (ONFH) were investigated.MethodsThe expression levels of HOTAIR and miR-17-5p in the mesenchymal stem cells (MSCs) derived from patients with non-traumatic ONFH and osteoarthritis (OA) were examined by real-time PCR. BMP-2 induced human MSCs from bone marrow (hMSC-BM) were used for osteogenic differentiation.ResultsIt was observed that the expression level of miR-17-5p was lower and the level of HOTAIR was higher in samples of non-traumatic ONFH compared with OA. HOTAIR downregulation induced by si-HOTAIR led to the increase of miR-17-5p expression and the decrease of miR-17-5p target gene SMAD7 expression. The values of osteogenic differentiation markers, including RUNX2 and COL1A1 mRNA expression and ALP activity, were also elevated by si-HOTAIR. However, the increase of these values was canceled by miR-17-5p inhibitor or SMAD7 upregulation.ConclusionHOTAIR played a role in regulating osteogenic differentiation and proliferation through modulating miR-17-5p and its target gene SMAD7 in non-traumatic ONFH.
Highlights
Osteonecrosis of the femoral head (ONFH) is a bone-destructive disease that mainly induced by the destruction of the blood supply and the disorder of coagulation and fibrinolysis system, leading to the collapse of femoral head
It was observed that the expression level of miR-17-5p was lower and the level of Homeobox transcript antisense RNA (HOTAIR) was higher in samples of non-traumatic ONFH compared with OA
HOTAIR played a role in regulating osteogenic differentiation and proliferation through modulating miR-17-5p and its target gene SMAD7 in non-traumatic ONFH
Summary
Osteonecrosis of the femoral head (ONFH) is a bone-destructive disease that mainly induced by the destruction of the blood supply and the disorder of coagulation and fibrinolysis system, leading to the collapse of femoral head. This disease commonly affects patients aged 30 to 50 years and makes a tremendous difference in the quality of life. A study revealed that miR-17-5p expression was significantly lower in non-traumatic ONFH than that in osteoarthritis (OA) samples, and functioned to facilitate the proliferation and differentiation of MSCs [8]. The relationship between long non-coding RNA HOTAIR and microRNA-17-5p (miR-17-5p) and their roles in osteogenic differentiation and proliferation in non-traumatic osteonecrosis of femoral head (ONFH) were investigated
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