Abstract
Emerging evidences have indicated that long non-coding RNAs (lncRNAs) are pivotal regulators of tumor development and progression. HNF1A-AS1 (HNF1A antisense RNA 1, C12 or f27) is a novel long non-coding RNA that acts as a potential biomarker and is involved in development and progression of several cancers. Nevertheless, we know nothing about the clinical significance and molecular mechanism of HNF1A-AS1 in bladder cancer. In this study, we found that HNF1A-AS1 is significantly up-regulated in bladder cancer. Further experiments had demonstrated that silencing HNF1A-AS1 in bladder cancer cells could inhibit the proliferation and induce apoptosis. Mechanistically, we found down-regulated of HNF1A-AS1 increased the expression of miR-30b-5p and subsequently inhibited the expression of Bcl-2, in a ceRNA-dependent way. Moreover, knockdown of miR-30b-5p reversed cell proliferation inhibition and cell apoptosis induced by silencing HNF1A-AS1. In conclusions, we demonstrated that HNF1A-AS1 plays an important regulatory role in bladder cancer and shed new light on lncRNA-directed diagnostic and therapeutics in bladder cancer.
Highlights
Bladder cancer is the ninth most common malignancy all over the world and it is estimated that there are 386,000 new cases are diagnosed worldwide annually [1,2,3,4]
HNF1A-AS1 was up-regulated in bladder cancer cell lines compared to corresponding normal urothelial cell line SV-HUC-1 (Figure 1E)
There are no specific symptoms for patents with bladder cancer at the early stage, some bladder cancers are found at advanced stage when treatments are less effective [35]
Summary
Bladder cancer is the ninth most common malignancy all over the world and it is estimated that there are 386,000 new cases are diagnosed worldwide annually [1,2,3,4]. The long non-coding RNAs (lncRNAs) are important new members of the ncRNA family with greater than 200 nucleotides in length [15, 16]. The rapid development of RNA genomics has highlighted the role of long non-coding RNAs (lncRNAs) in many human diseases, especially in cancers [17, 18]. Recent evidences have showed that many lncRNAs play important regulatory roles in diverse biological processes of cancers, such as UCA-1, HOTAIR, H19, MALAT1, ZEB1-AS1, PVT-1, PANDAR and etc [19,20,21,22,23,24,25]. HNF1A-AS1 (HNF1A antisense RNA 1) is a novel identified lncRNA with 2455 nucleotides in length and localized at the chromosome 12 [26]. The biological function and underlying mechanism of action of HNF1AAS1 in bladder cancer is completely unknown
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