Long non-coding RNA HLA complex group 18 promotes gastric cancer progression by targeting microRNA-370-3p expression.

Abstract

Our research was aimed at investigating the biological character of human leukocyte antigen complex group 18 (HCG18) on gastric cancer (GC) progression and its potential mechanisms. The expression characteristics and prognostic values of HCG18 in GC were evaluated through the GEPIA database and Kaplan-Meier plotter database. Quantitative real-time PCR and Western blot were used for quantification of messenger RNA expression, microRNA (miRNA) expression and protein expression. Cell proliferation, migration and invasion were detected by cell counting kit-8 assay, 5'-bromo-2'-deoxyuridine assay and Transwell assay, respectively. Dual-luciferase reporter gene assay and RNA immunoprecipitation assay were used for examination of the interactions among HCG18, miR-370-3p and epidermal growth factor receptor (EGFR) 3'UTR. HCG18 expression was up-regulated in GC tissues, and its high expression was closely associated with increased tumour size, advanced TNM stage, poor differentiation of tumour tissues and unfavourable prognosis of patients with GC. Additionally, HCG18 overexpression promoted the proliferation, migration and invasion of GC cells, and its knockdown suppressed the malignant phenotypes of GC cells. Furthermore, HCG18 served as a miRNA sponge to repress miR-370-3p and indirectly up-regulated EGFR expression in GC cells. HCG18 served as a tumour-promoting factor in GC progression by modulating the miR-370-3p/EGFR axis.