Long Non-Coding RNA H19 Expression in Leukemia Patients

Abstract

Abstract Objective: BCR-ABL fusion gene occurs with the formation of translocations in the t(9;22) region of the Philadelphia (Ph) chromosome, which is used as a diagnostic biomarker in Chronic Myeloid Leukemia (CML). These abnormal genetic changes, which cover 15% of leukemias, reach dimensions that threaten human life. Recent studies have determined that thousands of genes expressed in differentiation and development processes contain non-protein-coding RNA with a regulatory role. Of these, the first discovered long noncoding RNA (LncRNA) H19 has been associated with its biological role, cell proliferation, apoptosis and metastasis Therefore, studies have been conducted considering that LncRNA H19 can be used as a biological marker in CML patients. Method: For this study, blood from 72 CML patients over 18 years of age and 64 healthy individuals were used. After RNA isolation of each of these bloods and cDNAs were obtained, the expression levels of the LncRNA H19 gene were analyzed by Real Time PCR method. Results: As a result of the expression study, it was found that LncRNA H19 gene expression increased 4.37 times and was upregulated in Bcr-Abl positive patients (p=0.414683). Conclusion: In this study conducted in Diyarbakır region, we think that LncRNA H19, which is up-regulated in terms of CML profiles, can be used as a biological marker for new treatment applications.