Abstract
Long non-coding RNA (lncRNA) FYVE, RhoGEF and PH domain containing 5 antisense RNA 1 (FGD5-AS1) has been reported as an oncogene in colorectal cancer, promoting its tumorgenesis. The present paper focused on searching the potential function of FGD5-AS1 in non-small cell lung carcinoma (NSCLC). There are connections between the expression of lncRNA FGD5-AS1 and human NSCLC tumor growth and progression. Also, the relationships between FGD5-AS1, hsa-miR-107 and mRNA fibroblast growth factor receptor like 1 (FGFRL1) are going to test their interaction in NSCLC cell lines, which may cause a series of biological behaviors of NSCLC cells. qRT-PCR analysis was conducted to test the expression of RNAs in different situation. CCK-8 experiment and clone formation assay were performed to assess proliferation of NSCLC cells. Also, connection between FGD5-AS1 and hsa-miR-107 were investigated by luciferase reporter assay and RNA pull-down assay. Rescue experiments were performed to verify the modulating relationship between FGD5-AS1, hsa-miR-107 and FGFRL1. High-level expression of FGD5-AS1 was found in NSCLC. FGD5-AS1 may promote the proliferation of NSCLC cells. Also, the combination between hsa-miR-107, FGD5-AS1 and NSCLC have been proved, which means they can play an interaction function in NSCLC cells. Thence, we concluded that lncRNA FGD5-AS1 promotes non-small cell lung cancer cell proliferation through sponging hsa-miR-107 to up-regulate FGFRL1.
Highlights
The situation of death caused by cancer is appalling, and one of the most malignant cancers is lung cancer [1]
Long non-coding RNA (LncRNA) FGD5-AS1 was highly expressed in non-small cell lung carcinoma (NSCLC) tissues and cells, and FGD5-AS1 promoted NSCLC cells’ proliferation
Overall survival of NSCLC patients according to high and low expression of FGD5-AS1 was detected in Figure 1C, and we found the patients with high expression of FGD5-AS1 have a relative lower overall survival than the patients with low expression of FGD5-AS1
Summary
The situation of death caused by cancer is appalling, and one of the most malignant cancers is lung cancer [1]. Non-small cell lung cancer is most deaths causing cancer, which accounts for approximately 80% of all lung cancers, and approximately 75% of patients are found to be in the advanced stage, resulting in a low 5-year survival rate. There are some examples to prove this fact. The 5-year survival rate of non-small cell lung carcinoma (NSCLC) is only 11%, which means most patients can not get completely cured after their therapy [2]. Tumor invasion and metastasis are the major problems in disease progression and therapy failure, which cause immense cases of decease [3]. Thence, a strategy of prevention from tumor metastasis is really essential
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