Abstract
1. 1. Pig α-fetoprotein (AFP) and albumin were isolated from fetal serum by DEAE-Sephadex ion exchange chromatography combined with Cibacron Blue-Sepharose and trypsin-Sepharose adsorptions. 2. 2. AFP, fetal albumin and adult albumin carried 2.6, 2.4, and 1.9 moles of fatty acids per mole of protein, respectively. 3. 3. Most of fatty acids bound to AFP were polyunsaturated; mainly arachidonic (20:4, n-6) and docosahexaenoic (22:6, n-3) acids, which accounted respectively for 21.7 and 18.8% of the total fatty acids. 4. 4. By contrast, the fatty acids found in the albumins (fetal and adult) were preferently saturated and monounsaturated. 5. 5. Arachidonic acid was a minor component in both albumins, and no docosahexaenoic acid was detected.
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