Locational relationship between corpus luteum and ovulatory follicle on ovaries alters follicular dynamics and progesterone concentrations of Thai indigenous beef cows exhibiting two follicular waves

Abstract

The present study aims to determine the impact of differences in the locational relationship between the previous corpus luteum (CL) and the further ovulatory follicle (OF) on follicular dynamics and progesterone (P4) concentrations in Thai indigenous beef cows (White Lamphun) exhibiting two follicular waves. Twenty-one cows, exhibiting the two-wave follicular pattern, were studied through interovulatory intervals (IOI), and classified according to the relationship between the previous CL and the further OF on the cattle model ovaries. Classifications were outlined as either an ipsilateral (same ovary) relationship (n = 12), or a contralateral (opposite ovaries) relationship (n = 9). Ultrasound monitoring, which evaluated the follicular diameter, and collection of blood for determining the P4 concentration were performed each day throughout the IOI. The IOI was longer (P < 0.05) in the contralateral cows than in the ipsilateral cows (19.7 ± 0.33 days vs 18.5 ± 0.29 days). Cows with an ipsilateral relationship were found to have further OFs with greater (P < 0.05) diameters than were cows with a contralateral relationship (13.9 ± 0.31 mm vs 12.1 ± 0.21 mm). The mean growth rate of the further OF was greater (P = 0.05) in the ipsilateral cows than in the contralateral cows (1.1 ± 0.11 mm/day vs 0.8 ± 0.04 mm/day). On Day 17 of the IOI, the ipsilateral cows demonstrated their lowest concentration of P4 (P < 0.05). On Day 18 of the IOI, the concentrations of P4 tended to be lower (P = 0.09) in the ipsilateral cows than in the contralateral cows (0.6 ± 0.04 ng/mL vs 1.1 ± 0.12 ng/mL). The interval from the luteinisation until the end of the luteolysis was longer (P < 0.05) in the contralateral group than in the ipsilateral group (18.5 ± 0.50 days vs 16.7 ± 0.33 days). Thus, we conclude that in Thai indigenous beef cows, the growth rate and diameter of the further OF during luteolysis increases more in the ipsilateral relationship than in the contralateral relationship.