Abstract
Citrobacter intermedius, Escherichia coli and Proteus morganii were converted into spheroplasts by adding lysozyme and EDTA to the sucrose medium. The periplasmic enzymes alkaline phosphatase and 5′-nucleotidase were released into the medium during spheroplasting, but the inducible tyrosine phenol-lyase and tryptophanase remained in the spheroplasts, as did the cytoplasmic positional markers glutamate dehydrogenase and glucose-6-phosphate dehydrogenase, indicating that both enzymes are located in the cytoplasm.
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