Abstract
The location of double bonds in polyunsaturated fatty acids is determined by gas chromatography-mass spectrometry after 4,4-dimethyloxazoline (DMOX) derivatization. A procedure for DMOX preparation, starting from fatty acid methyl esters (FAMEs) is proposed. The most interesting properties of these derivatives are presented especially for the analysis of long-chain polyenoic fatty acids. The use of DMOX derivatives in combination with FAMEs can be very useful for the identification of fatty acids in unknown samples.
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