Abstract

The sex pheromone glands (SPG) of Boarmia selenaria Schiffermuller were located and described by examining various ovipositor parts and appendages of this insect through the use of four successive techniques: electroantennography (EAG), gas-liquid chromatography (GLC), histology, and scanning electron microscopy (SEM). EAG and GLC evidence pointed to the area between the 9th and 10th segments on the ovipositor as the site of pheromone concentration. Subsequently, this localization was confirmed by histological and SEM examinations. Cross sections of the 9th-to-10th segment area revealed that the SPG resulted from folding of the epithelium. Most cells of this tissue were columnar, with large, round to oblong nuclei. Some cuboidal cells were also present. Major features revealed by SEM were two ventro-lateral sacs extending anterad within the lumen of the intersegmental membrane; and longitudinal striation of the 9th and 10th segments, with only the anterior one showing spines protruding between striations.

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