Abstract
AbstractThis is an interesting procedure from late Pont-Lezica. He demonstrated that it is possible to show the exact location of proteins, nucleic acids, carbohydrates, and small molecules in a tissue using a simple tissue printing method, which makes tissue prints. This method uses plant tissues to produce prints on a nitrocellulose membrane to show amazing anatomical details even without staining. Such tissue printing can be used to record developmental changes in tissues over time. The protocol given here is based on the method published by the late Pont-Lezica. It shows a widely used application to localize proteins and glycans (biomolecules with carbohydrate molecules attached) with antibodies or lectins. Also, one can use this method to localize other molecules.KeywordsImmunodetection Tissue blotting Protein localization Western blotting
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