Abstract
Localized surface plasmon resonance (LSPR) has been extensively studied as potential chemical and biological sensing platform due to its high sensitivity to local refractive index change induced by molecule adsorbate. Previous experiments have demonstrated the LSPR generated by gold nanoholes and its biosensing. Here, we realize large uniform area of nanoholes on scale of cm2 on glass substrate by nanosphere lithography which is essential for mass production. The morphology of the nanoholes is characterized using scanning electron microscope and atomic force microscope. The LSPR sensitivity of the nanoholes to local refractive index is measured to be 36 nm/RIU. However, the chip has demonstrated high sensitivity and specificity in biosensing: bovine serum albumin adsorption is detected with LSPR peak redshift of 27 nm, and biotin-streptavidin immunoassay renders a LSPR redshift of 11 nm. This work forms a foundation toward the cost-effective, high-throughput, reliable and robust chip-based LSPR biosensor.
Highlights
Localized surface plasmon resonance (LSPR) is coherent oscillation of conduction electrons confined in noble metallic nanoparticles which is excited by electromagnetic radiation [1–3]
The chip has demonstrated high sensitivity and specificity in biosensing: bovine serum albumin adsorption is detected with LSPR peak redshift of 27 nm, and biotin-streptavidin immunoassay renders a LSPR redshift of 11 nm
The potential of LSPR as biosensing platform relies on the fact that the resonance frequency and intensity of LSPR are highly sensitive to the material, shape, size and local dielectric constant of the metal nanoparticles [5, 6]
Summary
Localized surface plasmon resonance (LSPR) is coherent oscillation of conduction electrons confined in noble metallic nanoparticles which is excited by electromagnetic radiation [1–3]. Detection of bovine serum albumin (BSA) binding and biotin-streptavidin immunoassay to gold nanoholes are realized, with LSPR extinction peak red-shifts 4-fold higher than the previously reported nanohole experiments in BSA test [10].
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