Localized morphometric deformations of small airways and alveoli in intact mouse lungs under quasi-static inflation

Abstract

Localized morphometric deformations of small airways and alveoli during respiration have several biomechanical and physiological implications. We developed fast synchrotron radiation CT system to visualize the small airways and alveoli of an intact mouse lung without fixation and dehydration, and analyzed their localized morphometric deformations between functional residual capacity (FRC) and total lung capacity (TLC). The maximum resolution of 32.6lp/mm at the 5% modulation transfer function level can be achieved with 11.8-microm voxels and 7-min scanning. Compared with the values at FRC, the diameter and length for smaller airways (diameter at FRC <200 microm) increased by 68.8% and 29.5% (averaged value), and those for larger airways (diameter at FRC >400 microm) increased by 45.2 and 22.9% (averaged value), at TLC. Moreover we defined the volume behavior as the percentage of airway volume at FRC for TLC. The volume behavior for the small airways was not similar to that of the lung volume. These results indicated that all airways did not behave homogenously.