Localized and discrete changes in neuropeptide (LHRH and TRH) and neurotransmitter (NE and DA) concentrations within the olfactory bulbs of male mice as a function of social interaction

Abstract

Individually housed male mice were exposed to either an intact male or an ovariectomized female mouse for 1 min and decapitated at 5, 15, or 60 min to examine the hypothesis whether discrete changes in olfactory bulb neuropeptide (LHRH and TRH) and neurotransmitter (NE and DA) concentrations would occur following onset of exposure. A nonexposed control group (decapitated at time 0) was also included. Bilateral olfactory bulbs were dissected into anterior dorsal (ADOB) and posterior dorsal (PDOB) olfactory bulb fragments and prepared for radioimmunoassays (LHRH and TRH) or radioenzymatic assays (NE and DA). Concentrations of LHRH and NE, but not of TRH and DA, from the PDOB were significantly greater than those of ADOB fragments. Exposure to a male resulted in a significant increase of PDOB LHRH at 5 min following exposure and a significant increase in LHRH at 15 min following female exposure. Norepinephrine within the ADOB and PDOB and DA within the PDOB demonstrated a statistically significant increase at 60 min following exposure to an ovariectomized female. In marked contrast, no statistically significant changes were obtained following male exposure. These results not only demonstrate a preferential localization of neuroregulators within the olfactory bulb of male mice but discrete changes in the concentration of these neuroregulators in response to male or female exposure, suggesting the possibility that some processing and coding of chemical cue information during social encounters already occurs at the level of the olfactory bulb.