Year-end Sale % OFF 
Abstract
The blood–cerebrospinal fluid barrier (BCB) is important in maintaining brain manganese (Mn) homeostasis. This barrier consists of a single layer of epithelial cells, connected by tight junctions, that restrict the passage of nutrients to only allow molecules to be carried through the membrane by a transporter. These epithelial cells are polarized with asymmetrical blood-facing and cerebrospinal fluid-facing sides. Here, we have established a polarized model of a human choroid plexus papilloma cell line, HIBCPP. For the first time, Mn importers ZIP14 and ZIP8 were identified in HIBCPP cells and were found to be enriched at the basolateral and apical sides of the cell monolayer, respectively. The localization of each ZIP protein adds to the understanding of Mn transport across the HIBCPP BCB model to help understand the mechanism of Mn homeostasis within the brain.
Highlights
The central nervous system is dependent on a controlled environment to function optimally.This environment is established through the passive and active transport mechanisms within the blood–brain barrier (BBB) and the blood–cerebrospinal fluid barrier (BCB)
We identified that both ZIP14 and ZIP8 are expressed in HIBCPP cells and that both transporters are involved in cellular Mn uptake
To determine whether ZIP14 and ZIP8 are expressed in HIBCPP cells, we first aimed to use Quantitative Real-Time PCR (qRT-PCR) analysis to measure the expression levels of these two genes
Summary
The central nervous system is dependent on a controlled environment to function optimally This environment is established through the passive and active transport mechanisms within the blood–brain barrier (BBB) and the blood–cerebrospinal fluid barrier (BCB). The BCB exists at the choroid plexus, a single cell layer of choroid plexus epithelial cells that are connected to each other by tight junctions and anchored to a basement membrane surrounding blood vessels. It is found in all four ventricles of the brain. The choroid plexus is responsible for the secretion of CSF, as well as the exchange of various factors and nutrients between the CSF and the blood [4]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
