Abstract
Phospholipase C-β1 (PLC-β1), one of the PLC-β isozymes, exists as two immunologically distinguishable polypeptides of 150 (PLC-β1a) and 140 kDa (PLC-β1b) which are encoded in two distinct transcripts and generated by alternative splicing of a single gene. In this study, the subcellular localization of the two phospholipases C-β1 proteins was examined in rat C6Bu-1 glioma cells using immunological techniques. Immunoblot analysis revealed that the two forms of PLC-β1 were detectable in both cytosolic and nuclear fractions. PLC-β1a appeared to be located preferentially in the cytosol, whereas PLC-β1b was found predominantly in the nuclei of C6Bu-1 cells. Immunocytochemical experiments confirmed the differential localization of the two PLC-β1 species in C6Bu-1 cells. These results suggest that the two PLC-β1 proteins may have different physiological roles in the cell.
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