Localization of transglutaminase activity in type II epithelial cell cultures and elevation of enzyme activity in lungs of rats instilled with quartz.

Abstract

Transglutaminase activity, assessed by the incorporation of [14C]-putrescine into N-acetylated dephosphorylated beta-casein, was not detectable in sonicates of alveolar macrophages and fibroblasts but was located in all preparations of rat alveolar type II cells. Enzyme activity was induced in these cells up to 7 days in vitro but not stimulated further by the direct addition of quartz to the cultures. Transglutaminase activity in whole lung sonicates increased significantly after short-term exposure to DQ-12 quartz. An increase in the numbers of type II cells and subsequent release of activated transglutaminase, concomitant with a quartz-induced elevation in lung calcium levels and potential protein substrates, is likely to lead to an increase in protein crosslinking both in the alveolar space and interstitium.