Abstract
The replication site of DNA in HeLa S3 cells has been studied with “M-band” technique after Tremblay and others. When the cells are mixed with sodium lauroyl sarcosinate and Magnesium ion directly on the sucrose gradient and spun, virtually all the DNA is caught in the M-band, suggesting that mammalian cell DNA is attached to the nuclear membrane. After being treated with sodium lauroyl sarcosinate and sheared by a vortex-mixer, the DNA is found to have moved from the M-band to the supernatant. Pulse-labeled DNA is more resistant to shearing as compared with the bulk of DNA and chased away from the M-band during a subsequent growth. These results indicate that, as in the case of bacteria, the replication points of mammalian cell DNA are on the membrane.
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