Abstract
The human α-globin gene cluster has been assigned previously to chromosome 16 using a molecular hybridization assay of somatic cell hybrids. We have carried out a regional assignment to the upper half of the short arm of chromosome 16 in the region 16p12-pter by hybridization in situ of α-globin-specific probes to fixed metaphase chromosomes. Suitable hybridization probes, of sufficient length to give a detectable signal, were prepared free from highly repetitive sequences, by the isolation of restriction enzyme fragments from genomic recombinant clones covering the ζ, ψα and α-globin gene regions of the chromosome. A total of 5.2 × 10 3 bases of hybridized sequence was used to detect the α-globin gene cluster. The criteria of chromosome specificity, overall efficiency of hybridization and regional localization were used to establish the localization. These criteria have been applied previously to the mapping of the β-globin gene cluster.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
