Abstract
The peritoneum consists of a monocellular layer of mesothelium supported by fibrous connective tissue of variable thickness. This tissue covers the walls of the abdominal cavity (parietal) and the surfaces of the abdominal organs, omentum and mesentery (visceral). A recent study has shown that the mesothelium synthesizes phosphatidylcholine which is the principle constituent of pulumonary surfactant phospholipids (1). Pulmonary surfactant is synthesized by Type II pneumocytes and stored in cytoplasmic structures called lamellar bodies. Tannic acid fixation has been shown to enhance preservation of these lamellar bodies (2). In the present study, samples of peritoneum from mice were prepared by tannic acid fixation and examined.CF1 mice were injected intraperitoneally with 1% tannic acid in a 2% glutaraldehyde solution (Sorensen's phosphate buffer). Tissues from the parietal peritoneum (anterior abdominal wall) and mesentery were removed and placed in the tannic acid/glutaraldehyde solution for 24 hours at 4°C. Tissues were post-fixed in 1% osmium tetroxide and embedded in Spurr.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
