Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia

Abstract

Sulfated glucuronyl glycolipids (SGGLs) in human dorsal root ganglion (DRG)and sympathetic ganglion (SG) were analyzed biochemically and immunohistochemically. SGGLs were enriched in human DRG ( 1.02 ± 0.23 μg/mg protein), whereas much lower concentrations of these glycolipids ( 0.043 ± 0.23 μg/mg protein) were detected in SG. Myelin within DRG and SG was immunostained by anti-SGGL antiserum, although only a few myelinated fibers were seen in SG. Nerve cell bodies or unmyelinated fibers were not immunostained. Subcellular fractionation study of human DRG demonstrated that these glycolipids were not only enriched in myelin but also in the axolemma-enriched fraction. These data are consistent with the view that SGGLs may be expressed on myelinated fibers in myelin and axolemma, suggesting that these compounds may play an important role in regulating myelinogenesis.