Localization of Serine Kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), Specific for the SR Family of Splicing Factors in Mouse and Human Chromosomes

Abstract

The serine- and arginine-rich (SR) splicing factors play an important role in both constitutive and alternative pre-mRNA splicing, and the functions of these splicing factors are regulated by phosphorylation. We have previously characterized SRPK1 (SFRSK1) and SRPK2 (SFRSK2), which are highly specific protein kinases for the SR family of splicing factors. Here we report the chromosomal localization of the mouse and human genes for both kinases. SRPK1 probes detected two loci that were mapped to mouse Chromosomes 17 and X using The Jackson Laboratory interspecific backcross DNA panel, and SRPK2 probes identified a single locus on mouse Chromosome 5. Using a somatic cell hybrid mapping panel and by fluorescencein situhybridization, SRPK1 and SRPK2 were respectively mapped to human chromosomes 6p21.2–p21.3 (a region of conserved synteny to mouse Chromosome 17) and 7q22–q31.1 (a region of conserved synteny to mouse Chromosome 5). In addition, we also found multiple SRPK-related sequences on other human chromosomes, one of which appears to correspond to a SRPK2 pseudogene on human chromosome 8.