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Abstract
The enzymes of the CDP-choline pathway of lecithin biosynthesis are present in etiolated hypocotyl hooks of bean seedlings ( Phaseolus vulgaris L.). The subcellular distribution of the enzymes was determined by sucrose density gradient centrifugation. The enzyme choline kinase (EC 2.7.1.32) proved to be soluble protein while phosphorylcholine-cytidyl transferase (EC 2.7.7.15) and phosphorylcholine-diglyceride transferase (EC 2.7.8.2) were associated with particulate fractions. Phosphorylcholine-cytidyl transferase activity was found in microsomal fractional (density 1 · 10 g cm −3) whereas phosphorylcholine-diglyceride transferase activity was exclusively localized in the microsomal fraction.
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