Abstract

Chrysosplenium americanum Schwein. ex Hooker (Saxifragaceae) accumulates a variety of partially methylated flavonol glucosides. Specific antibodies to tri- and tetramethylated flavonol-2′- O-glucosides, located using fluorescein isothiocyanate (FITC) goat antirabbit antibody, were used for the localization of the flavonol glucosides in leaf epidermis, cross sections and protoplasts. The results indicated that flavonoid accumulation occurred mainly in the walls of epidermal cells and, to a much lesser extent, in mesophyll cell walls. The weak fluorescence observed in the vacuoles of protoplasts suggested a minor role of this compartment in the accumulation process. The significance of flavonoid deposition within epidermal cell walls is discussed in relation to the lipophilic nature of these compounds and their possible role in the physiology of the plant.

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