Abstract
The localization of nitrogen within the leaf cavities of Azolla filiculoides Lam., where Anabaena azollae Strasb. and bacteria are living, had been obtained by means of electron spectroscopic imaging (ESI) performed on ultrathin unstained sections of different leaves. A net distribution of nitrogen localized inside the epidermal hairs of Azolla, the vegetative cells and heterocysts of A. azollae and bacteria has been visualized by image analysis. By electron energy loss spectroscopy (EELS) nitrogen peaks have been evidenced in spectra recorded in the observed areas.
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