Abstract
An antibody raised against the inactive (non-activated) form of NADP-linked malate dehydrogenase (MDH) from corn leaves precipitated the enzyme and inhibited its catalytic activity. The antibody had no significant effect on the low levels of NAD-linked activity in the preparation. When applied in enzyme-linked immunosorption assay (ELISA) and immunofluorescence procedures, the antibody revealed that NADP-MDH is located exclusively in chloroplasts of mesophyll cells. The evidence also indicated that NADP-MDH undergoes a conformational change when activated. The results demonstrate that immunological methods can be used to determine the cellular location of C 4 enzymes both in vitro and in situ.
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