Abstract
An antibody raised against the inactive (non-activated) form of NADP-linked malate dehydrogenase (MDH) from corn leaves precipitated the enzyme and inhibited its catalytic activity. The antibody had no significant effect on the low levels of NAD-linked activity in the preparation. When applied in enzyme-linked immunosorption assay (ELISA) and immunofluorescence procedures, the antibody revealed that NADP-MDH is located exclusively in chloroplasts of mesophyll cells. The evidence also indicated that NADP-MDH undergoes a conformational change when activated. The results demonstrate that immunological methods can be used to determine the cellular location of C 4 enzymes both in vitro and in situ.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.