Abstract
Localization of metmyoglobin-reducing enzyme system components (NADH-cytochrome b 5 reductase, cytochrome b 5 outer mitochondrial membrane cytochrome b) was demonstrated in bovine skeletal muscle by immunohistochemical techniques. Both NADH-cytochrome b 5 reductase and OM cytochrome b were located in red fibers. However, localization of cytochrome b 5 did not show a definite difference between fiber types. Using an immunoblotting technique. NADH-cytochrome b 5 reductase was found predominantly in the mitochondrial fraction, but it was also detected at lower levels in the microsomal fraction. OM cytochrome b was found predominantly in the mitochondrial fraction, but cytochrome b 5 was detected only in the microsomal fraction. The results from this study, along with previous work about the localization of myoglobin in muscle, suggest that NADH-cytochrome b 5 reductase reduces metmyoglobin by using OM cytochrome b at the mitochondrial surface and, in part, by using cytochrome b 5 at the sarcoplasmic reticulum.
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