Abstract
Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated. Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50–70% of the enzyme was localized close to the cytoplasmic membrane, which implies “facultative” membrane association of the enzyme. This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms.
Highlights
Methyl-coenzyme M reductase (MCR) is the key enzyme of the final, methane-forming step in methanogenesis
The enzyme catalyses the reductive cleavage of methylcoenzyme M (CoM-S-CH3) using coenzyme B (HS-CoB) as reductant which results in the production of methane and the heterodisulfide CoM-S-S-CoB
In Methanobacterium thermoautotrophicum, two different localizations of the MCR could already be shown [13]. We show that these results are true for other methanogens, and we will discuss these results in view of immunolocalization of the key enzyme MCR for studies in environmental biofilms
Summary
Methyl-coenzyme M reductase (MCR) is the key enzyme of the final, methane-forming step in methanogenesis. Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated.
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