Localization of met-enkephalin and vasoactive intestinal polypeptide in the brains of male zebra finches.

Abstract

An interconnected series of brain nuclei controls song learning and behavior in male zebra finches (Poephila guttata). This study examined the distribution of fibers, terminals, and somata immunoreactive for two neuropeptides, methionine-enkephalin (ENK) and vasoactive intestinal polypeptide (VIP), in song-control nuclei of adult males. In addition, the broad pattern of major regions of labeling throughout the forebrain and midbrain was determined. The telencephalic song-control nuclei MAN (magnocellular nucleus of the anterior neostriatum), Area X of the striatum, HVC (higher vocal center), and RA (robust nucleus of the archistriatum) contained abundant ENK immunoreactivity, including labeled fibers and somata. In addition, intensely labeled fibers and terminals were seen in the thalamic nucleus DLM (medial portion of the dorsolateral nucleus of the anterior thalamus). High levels of VIP immunoreactivity were also seen in MAN, HVC, and RA, but this label consisted of fiber and terminals only. Area X and surrounding striatum contained extremely sparsely distributed VIP-labeled processes. Somata positive for VIP were not seen throughout cortical regions such as the neostriatum and hyperstriatum but were abundant in the lateral striatum (paleostriatum augmentatum, PA) and may contribute to a dense field of terminal labeling seen in the globus pallidus. The apparent presence of a robust VIP-positive striato-pallidal projection is not typical of major basal ganglia pathways in vertebrates, raising the possibility that passerine birds have diverged from the typical amniote pattern.