Abstract
Summary Localization of polyphosphate-containing bodies in vacuoles and yeast cell cytoplasm was demonstrated, using electron microscopy with cytochemical staining. By the same procedure condensed phosphate-containing particles were detected in the nucleus, in the area of cytoplasmic membrane, and on the cell surface. The presence of these compounds on the surface of initial cells was confirmed by toluidine blue solution metachromasy and by scanning electron microscopy. At the dehydration of yeast cells the increase in the body number in the cytoplasm and yeast cell envelope and decrease in their amount in vacuoles was observed. The leakage of a part of cell phosphorous compounds in the medium was established at glutaraldehyde fixation of initial and dehydrated cells. The fixation of dehydrated yeast cells in the presence of lead ions revealed the sites of phosphorous compounds, released from cells, localized in a distinct area of the cell wall.
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