Localization of immunoreactive regions in the beef heart adenine nucleotide carrier using rabbit antisera against the carboxyatractyloside-liganded and the sodium dodecyl sulfate denatured carrier forms

Abstract

The existence of different antigenic determinants in the beef heart adenine nucleotide (AdN) carrier was demonstrated by exploring the reactivity of fragments of the carrier protein with rabbit antisera directed against either the beef heart AdN carrier denatured with sodium dodecyl sulfate (NaDodSO4 carrier) or the beef AdN carrier liganded by the specific inhibitor carboxyatractyloside (CATR-carrier). The antigenic determinants reacting with antiserum to the CATR-carrier appeared to be close to the N- and C-terminal ends of the carrier protein, whereas those reacting with antiserum to the NaDodSO4 carrier were located preferentially in the central region of the protein. The same antisera were used to study the immunogenic specificity of the beef liver AdN carrier, the rat heart AdN carrier, and the rat liver AdN carrier. The beef liver and rat heart AdN carriers were found to react with both antisera whereas the rat liver AdN carrier reacted essentially with the CATR-carrier antiserum.