Abstract
Testis is the tissue with immune privilege which is maintained by blood-testis barrier to protect autoimmunogenic components of testis. IgG have binding affinity for CD16, CD32 and CD64 receptors present on various immune cells. Present study was conducted to investigate the IgG antigenic sites in the testicular tissue of goat. The paraffin embedded histological sections were processed for immunohistochemistry protocol using Anti Mouse IgG FITC conjugate antibodies and observed under fluorescence microscope. Strong reaction was observed in epithelial lining of seminiferous tubule and moderate reaction was observed in lumen of seminiferous tubules and interstitial space. Present studies indicate that the most of IgG antigenic components i.e. CD16, CD32 and CD64 receptors were located in these sites. Current work will help in understanding of immune privilege and provide a baseline on which further studies can be conducted.
Highlights
Testis is the tissue with immune privilege which is maintained by blood-testis barrier
The fluorescence was observed in the interstitial components and in epithelial lining and lumen of seminiferous tubules indicates that the most of IgG antigenic immunological componentsi.e.CD16, CD32 and CD64 receptors were located in these sites
Frequency of fluorescence is higher in epithelial lining of seminiferous tubule indicated that most of the CD16, CD32 and CD64 receptors were located on this site
Summary
Testis is the tissue with immune privilege which is maintained by blood-testis barrier. This blood-testis barrier protects the various autoimmunogenic components of testis like spermatozoa which the body’s immune system recognizes as foreign (Terayama et al, 2014). Various types of immune cells have receptors for the IgG. CD16 receptor present on the NK cells, macrophages, subpopulation of T-cells, immature thymocytes , neutrophils and stimulated eosinophils has binding affinity for IgG (Owen et al, 2013). CD32 receptor present on B cells, monocytes, macrophages, dendritic cells, and on endothelial cells binds with Fc portion of IgG (Owen et al, 2013). In addition CD64 present on many immune cells have high affinity for IgG (Owen et al, 2013).
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