Localization of human myeloid-associated surface antigen detected by a panel of 20 monoclonal antibodies to the q12-qter region of chromosome 11.

Abstract

Human x mouse myeloid cell hybrids were obtained after fusion of human leucocytes and the murine myeloid cell line WEHI-TG. The hybrids were tested for reactivity with a panel of monoclonal antibodies with a known myelocytic, monocytic, or myelomonocytic specificity. Twenty antibodies, all of which bind specifically to the surface of human myeloid cells, exhibited very similar reactivity patterns with the hybrid clones. Chromosomal analysis of hybrid cell metaphases revealed that the gene(s) involved in the expression of the antigen(s) recognized by these antibodies must be located on human chromosome 11 in the region q12-qter. These results are compatible with the evidence obtained from other studies that several, if not all, of the myeloid-specific antibodies used are reactive with a similar antigenic determinant present on human myeloid cells.