Abstract
A new method for the localization of human chorionic gonadotropin (HCG) in monolayer cultures of human trophoblast cells is described. This is based on the principle of mixed agglutination with the use of HCG-coated human erythrocytes as the indicator cells. Both cytotrophoblasts and syncytiotrophoblasts were found to produce HCG in vitro. Seven-day primary cultures from placental villi were found to contain many of these HCG-producing cells. HCG was not demonstrable by this method on trophoblast cells which had been removed from the culture flasks by trypsin.
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