Abstract
The distribution of ganglioside GM1 in primary cultured cells from rat cerebral hemispheres was studied. These dissociated cell cultures are capable of forming myelin. 2′,3′-Cyclic nucleotide 3′-phosphodiesterase (CNPase) activity, which is considered to be a good marker for myelin, increased with the onset of myelin formation. Ganglioside GM1 was localized on the myelin-related structures using a biotinylated choleragen and avidin peroxidase technique. The distribution of ganglioside GM1 resembled that of immunostained CNPase.
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