Localization of galanin immunoreactivity in the opossum esophagus

Abstract

Galanin-like immunoreactivity was studied at 7 levels of the opossum esophagus, lower esophageal sphincter (LES) and adjacent portion of the stomach by indirect immunofluorescence; it was restricted to nervous structures. The majority of myenteric and submucous neurons were galanin-positive and received positive axo-somatic terminations. They also sent out axons staining positively. Galanin-positive fibers and a few atypically located neurons formed a mucous plexus at the bases of mucous glands. Varicose galanin fibers innervated the muscularis mucosae, circular and longitudinal muscle layers, while thick fascicles traversed the muscularis mucosae and circular muscle, possibly interconnecting the myenteric, submucous and mucous plexuses. Galanin-positive fibers did not supply blood vessels. There was no obvious gradient of innervation density along the esophagus, but the sphincter appeared to be more densely innervated than the esophageal body. There was no galanin-positive input to striated muscle. In view of its widespread distribution, this neuropeptide may serve multiple functions in the esophagus.