Localization of Fe‐containing superoxide dismutase in cyanobacteria from the Baltic Sea: depth and light dependency

Abstract

The abundance and cellular location of Fe‐containing superoxide dismutase (Fe‐SOD) in trichomes of Nodularia, Aphanizomenon and Anabaena collected from various depths in the Baltic Sea, and in trichomes of a cultured Nodularia strain, BC Nod‐9427, isolated from the Baltic Sea, was examined by immunogold labelling. For trichomes collected from natural populations the areal concentration of Fe‐SOD labelling decreased with depth: trichomes collected from surface accumulations had between 8 and 11 gold particles μm−2 whereas trichomes collected from a depth of 18 m were unlabelled. When trichomes collected from a depth of 10 m (mean areal labelling density 0·5 gold particles μm−2) were exposed to the higher irradiances present at 1 m, the areal concentration of Fe‐SOD increased to 3·5–4 gold particles μm−2 within 4 h. When cultures of Nodularia strain BC Nod‐9427, adapted to low light (10 μmol m−2 s−1), were transferred to an incident irradiance of 1350 μmol m−2 s−1, a doubling of the areal concentration of Fe‐SOD gold label was observed within 1 h. Addition of 3‐(3,4‐dichlorophenyl)‐1,1′‐dimethylurea (DCMU) to cultures immediately before their transfer to increased illumination resulted in a decrease in areal Fe‐SOD concentrations whereas addition of CdCl2 caused an increase over and above that induced by the elevated irradiance. These results suggest that Baltic Sea cyanobacteria are able to modulate their Fe‐SOD content but that this might be in response to oxidative stress rather than to light per se.