Localization of Estrogen Receptor α and β RNA in Germinal and Nongerminal Epithelia of the Channel Catfish Testis

Abstract

The germinal epithelium of the anterior region of the channel catfish testis is the site of spermatogenesis, whereas the nongerminal epithelium of the posterior region (seminal vesicle) may play a role in the regulation of seminal fluid composition. The available information indicates that estrogens play a physiological role in the regulation of spermatogenesis and seminal fluid composition in vertebrates. However, the cellular distribution of estrogen receptor (ER) gene expression in the teleost testis is poorly understood. Therefore, the objective of this study was to determine the presence and cellular distribution of ERα and ERβ transcripts in germinal and nongerminal epithelia of the mature testis of channel catfish. RT–PCR of whole-testis RNA extracts showed that ERα and ERβ RNAs are present in the mature testis. In situ hybridization of histological sections of the germinal epithelium showed that primary spermatocytes contain little or no ERα or ERβ RNA. However, both ER transcripts were present in secondary spermatocytes and spermatids and their levels were relatively high in mature sperm. The columnar epithelium of the seminal vesicle also contained ERα and ERβ RNA. The ER RNAs in epithelial cells of the seminal vesicle were not evenly distributed throughout the cytoplasm but seemed to concentrate in their apical region, near the nucleus. In conclusion, ERα and ERβ genes are coexpressed in germinal and nongerminal epithelia of the mature testis of channel catfish and seem to be developmentally regulated in spermatocytes. These observations are consistent with the concept that estrogens, via interaction with ERα and ERβ, participate in the regulation of male gamete development and fertility.