Abstract
Localization of 2,4-dichloro- and 2,4-dibromophenol in multilamellar vesicles in a 1/1 dihalogenated phenol/lipid molar ratio was investigated by classical contrast variation X-ray scattering using the isomorphous replacement method. The results were compared with those obtained by anomalous small-angle X-ray scattering from a vesicle system doped with 2,4-dibromophenol. Dissimilarities in the results of the two methods are discussed, taking into account the advantages and disadvantages of both techniques in studying multilamellar systems.
Highlights
In the last few decades, studying the effect of different guest molecules on model membranes has turned to a high interest subject because of the biological relevance
The first goal of this work is to determine the localization of 2,4-dichloro- (DCP) and 2,4-dibromophenol (DBP) in a vesicle system made of 1,2-dipalmitoyl-snglycero-3-phosphatidylcholine (DPPC) and water in a 1/1 dihalogenated phenol/lipid molar ratio
If the concentration reaches the molar ratio of guest molecule/lipid = 0.6, the well ordered layer arrangement is recombined as Bragg peaks in higher order reappear
Summary
In the last few decades, studying the effect of different guest molecules on model membranes has turned to a high interest subject because of the biological relevance. It is known that a large number of phenolic compounds act on the basic cellular functions (Escher et al, 1999; Escher & Schwarzenbach, 1996; Sinclair et al, 1999). These molecules can evolve toxic effects without chemical reactions, but only by second order interaction while penetrating into the bilayer. The phenolic compounds studied are widely used in industry as flame retardants, biocides and wood treatment agents, and they have toxic effects on living organisms (Zhang et al, 2004; Olsen et al, 2002; Hassenklover et al, 2006)
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