Localization of alkaline phosphatase and proteins related to intercellular junctions in primary cultures of fetal rat hepatocytes

Abstract

The localization of alkaline phosphatase (ALP) and four proteins related to intercellular junctions in primary cultures of fetal rat hepatocytes was immunocytochemically investigated using fluorescence-labeled antibodies and confocal laser microscopy in order to determine whether the formation of intercellular junctions at the borders between adjacent rat hepatocytes becomes the trigger of translocation of ALP from the cytoplasm to the plasma membrane. Dexamethasone (DEX) which was supplemented in the base medium promoted the translocation of ALP from the cytoplasm to the plasma membrane surrounding bile canaliculus-like intercellular spaces and the appearance of connexin-32 at cell borders between adjacent fetal hepatocytes. E-cadherin, occludin and ZO-1 were localized at the cell borders between adjacent fetal hepatocytes irrespective of the presence of DEX. Occludin and ZO-1 were further localized along the plasma membrane surrounding bile canaliculus-like intercellular spaces formed by DEX. The present study indicates that the formation of adherens and tight junctions between adjacent rat hepatocytes does not become the trigger of ALP translocation from the cytoplasm to the plasma membrane, although we cannot be certain of whether the formation of gap junctions between adjacent rat hepatocytes triggers ALP translocation.