Abstract
The localization of adenovirus type 5 DNA replication has been investigated by both fractionation of isolated nuclei and electron-microscope autoradiography. Nuclear fractionation by means of the M-band-technique of Tremblay et al. (Tremblay, G. Y., Daniels, M. J., and Schaechter, M. (1969). J. Mol. Biol. 40, 65–76.) reveals that pulse-labeled adenovirus type 5 DNA cosediments with the nuclear membrane. On the basis of this finding it can be supposed that the site of viral DNA replication is at the nuclear membrane. However, electron-microscope autoradiography of infected cells shows that the pulse-labeled viral DNA is not located at the nuclear membrane but randomly distributed over the nucleus. It is concluded that the replication of adenovirus type 5 DNA occurs throughout the cell nucleus; the association of replicating viral DNA with a nuclear membrane complex should be considered as an artifact.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
