Abstract
AbstractThe cellular diversity of the lung, coupled with the unusual elasticity of this organ, provides unique challenges to the histotechnologist and the pathologist when attempting to identify specific cell types within areas of pulmonary inflammation. The morphological identification of alveolar macrophages, as a component of an inflammatory cell response within the lung, can be enhanced by selected histochemical and immunohistochemical characteristics. Intracellular enzymes that aid in the identification of macrophages include, but are not limited to, acid phosphatase, nonspecific esterase, and lysozyme. Airway perfusion of cold fixative, methacrylate embedment in the cold and histochemical techniques have been combined in order to optimize the evaluation of inflammatory lesions of rodent lungs. (The J Histotechnol 11:95, 1988.)
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