Abstract
The authors have previously reported that the DRA gene is located on chromosome 7. This assignment was based on Southern blot hybridization of a DRA cDNA to genomic DNA from rodent-human somatic cell hybrids. In this report, they localize the DRA gene to chromosome band 7q22-q31.1 by fluorescence in situ hybridization (FISH) with a full-length (2.9 kb) cDNA as probe. Metaphase spreads from normal human lymphocytes were prepared according to the method of Fan et al. The cDNA clone 611C was labeled with biotin-11-dUTP using a nick-translation kit (Oncor) followed by purification on a Sephadex G50-fine column. FISH and detection of immunofluorescence were performed according to the technique of Pinkel et al. with minor modifications. The chromosome preparations were stained with both diamidino-2-phenylindole and propidium iodide (Oncor) and observed with a Zeiss Axiophot fluorescence microscope. Hybridization was detected on chromosome 7 in 22 of 47 spreads examined. Of 89 fluorescent signals on all chromosomes, 44 (49%) were located on 7q. All signals on chromosome 7 appeared to be located at 7q22-q31.1. Hybridization is in the vicinity of the met protooncogene locus at 7q31.
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