Abstract
Cytoplasmic 3H-thymidine incorporated in oocytes of Schistosoma mansoni as seen on light microscope autoradiograms was removed by DNAase but not RNAase. On electron microscope autoradiograms, silver grains produced by 3H-thymidine were closely associated with mitochondria of oocytes. High resolution autoradiograms of 3H-actinomycin D, a compound which binds to nuclear and mitochondrial DNA, showed a majority of extranuclear silver grains was associated with mitochondria of oocytes. Exposure of worm sections to 4', 6-diamidino-2-phenylindole (DAPI), a compound that forms a fluorescent complex with DNA, produced a brilliant fluorescence in nuclei and cytoplasm of oocytes which was removed by DNAase but not RNAase. Incorporation of 3H-thymidine into nuclei and cytoplasmic components of oocytes was inhibited by two DNA-binding compounds, DAPI and acriflavin. This evidence indicted that the thymidine label emanated from mitochondrial DNA within the closely packed mitochondria of mature oocytes of S. mansoni.
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